Recipe

Ca2+/Mg2+-free Tyrode Ringer's saline

NaCl, 8.0 g

KCl, 0.3 g

NaH2PO4•5H2O, 0.093 g

KH2PO4, 0.025 g

NaHCO3, 1.0 g

Glucose, 2.0 g

Dissolve in 1 liter of H2O. Adjust pH to 7.6-7.7.

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  1. doi:10.1101/pdb.rec10357 Cold Spring Harb Protoc 2006: pdb.rec10357-

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