Recipe

Ligand-PEG-lipid

 
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0.05 mM ligand

methanol

Chloroform

Optional, please see Step 7.

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Ligand-PEG-lipid is 0.05 mM ligand (TAT peptide, sequence CGGGRKKRRQRRRGYG) - maleimide-functional PEG-lipid (1,2-distearoyl-sn-glycero-3-phosphoethanolamine- N-maleimide(polyethylene glycol 2000)) in methanol/chloroform [1/1, v/v]

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  1. doi:10.1101/pdb.rec10373 Cold Spring Harb Protoc 2006: pdb.rec10373-
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