Recipe

Denaturing anode stabilization media

Glycerol (14.5% w/w)

Hydroxypropylmethyl cellulose (HPMC) (0.12% w/w)

100 mM H2SO4

8 M urea

For 68.9 g = ~60 ml: Weigh out 6 g of H2SO4 (1 M). Add 9 g of H2O and 25 g of HPMC/glycerol/H2O stock (40%/40%/20% w/w/w). Add 28.9 g of urea. Dissolve by stirring in lukewarm water. Actual Consumption: ~10 g/hr.

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  1. doi:10.1101/pdb.rec10388 Cold Spring Harb Protoc 2006: pdb.rec10388-

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