Recipe

Denaturing cathode stabilization media

Glycerol (14.5% w/w)

Hydroxypropylmethyl cellulose (HPMC) (0.12% w/w)

100 mM NaOH

8 M urea

For 137.8 g = ~120 ml: Weigh out 12 g of 1 M NaOH. Add 18 g of H2O and 50 g of HPMC/glycerol/H2O stock (40%/40%/20% w/w/w). Add 57.8 g of urea. Dissolve by stirring in lukewarm water. Actual Consumption: ~20 g/hr.

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  1. doi:10.1101/pdb.rec10389 Cold Spring Harb Protoc 2006: pdb.rec10389-

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