Recipe

Microinjection buffer

 
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EDTA (0.5 M) (Sigma)

HCl (1 M)

Tris-Cl (1 M) (Sigma)

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Prepare a 5 mM Tris-Cl/0.1 mM EDTA solution with ultrapure H2O. Adjust pH to 7.4 with 1 M HCl. Filter through pre-washed 0.22 μm filter. Store at 4°C or -20°C.

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  1. doi:10.1101/pdb.rec10394 Cold Spring Harb Protoc 2006: pdb.rec10394-
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