Recipe

Solution I for agarose plugs

 
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~ 1 M Sorbitol (Merck, autoclaved)

20 mM EDTA (pH 8.0) (autoclaved)

14 mM β-mercaptoethanol (Merck)

2 mg/ml Zymolyase-20T (ICN 320921)

sterile H2O

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Prepare fresh, do not store.

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  1. doi:10.1101/pdb.rec10396 Cold Spring Harb Protoc 2006: pdb.rec10396-
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