Recipe

Solution III for agarose plugs

 
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~1 M Sorbitol (Merck, autoclaved)

20 mM EDTA (pH 8.0) (autoclaved)

10 mM Tris-Cl (pH 7.5) (autoclaved)

14 mM β-mercaptoethanol (Merck)

2 mg/ml Zymolyase-20T (ICN 320921)

sterile H2O

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Prepare fresh.

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  1. doi:10.1101/pdb.rec10398 Cold Spring Harb Protoc 2006: pdb.rec10398-
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