Recipe

Solution IV for agarose plugs

 
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1% Lithium dodecyl sulfate (Sigma L4632)

100 mM EDTA (pH 8.0) (autoclaved)

10 mM Tris-Cl (pH 8.0) (autoclaved)

sterile H2O

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Filter sterilize (0.22 micron). Store at room temperature.

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  1. doi:10.1101/pdb.rec10401 Cold Spring Harb Protoc 2006: pdb.rec10401-
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