Recipe

ES Cell lysis buffer

 
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100 mM Tris-Cl (pH 8.5)

5 mM EDTA

0.2% SDS

200 mM NaCl

100 μg/ml Proteinase K

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Store 20 mg/ml Proteinase K stock at -20°C and add to the lysis buffer prior to use.

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  1. doi:10.1101/pdb.rec10408 Cold Spring Harb Protoc 2006: pdb.rec10408-
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