Recipe

TS medium

 
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RPMI 1640, 500 ml

FBS, 130 ml (20% final)

Penicillin and Streptomycin (100x stock, 50 μg/ml each final)

Sodium pyruvate, 6.5 ml (100 mM stock, 1 mM final)

β-mercaptoethanol, 6.5 ml (10 mM stock, 100 μM final)

L-glutamine, 6.5 ml (200 mM stock, 2 mM final)

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  1. doi:10.1101/pdb.rec10416 Cold Spring Harb Protoc 2006: pdb.rec10416-
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