Recipe

Denaturing buffer containing guanidine hydrochloride

 
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6 M Guanidine-HCl

5 mM DTT

50 mM Tris-Cl (pH 7.5)

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Warmm the solution to 40ºC to dissolve the guanidine HCl. Adjust the pH to 7.5 if necessary.

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  1. doi:10.1101/pdb.rec10421 Cold Spring Harb Protoc 2006: pdb.rec10421-
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