Recipe

Laemmli sample buffer (2X)

Reagent Amount to add Final concentration (2X)
10% (w/v) SDS 4 mL 4%
Glycerol 2 mL 20%
1 M Tris-Cl (pH 6.8) 1.2 mL 120 mM
H2O 2.8 mL -
Add bromophenol blue to a final concentration of 0.02% (w/v). Store the 2X Laemmli sample buffer at room temperature.

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  1. doi:10.1101/pdb.rec10424 Cold Spring Harb Protoc 2006: pdb.rec10424-
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