Recipe

Bradford dye concentrate

 
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Coomassie Brilliant Blue G250, 100 mg

95% Ethanol, 50 ml

Concentrated phosphoric acid (H3PO4), 100 ml

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Add H2O to a final volume of 200 ml. The dye is stable at 4°C for at least 6 months. The dye concentrate is also available commercially from Bio-Rad.

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  1. doi:10.1101/pdb.rec10428 Cold Spring Harb Protoc 2006: pdb.rec10428-
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