Recipe

IMAC Tris elution buffer

 
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500 mM imidazole

500 mM NaCl

1 mM 2-Mercaptoethanol

20 mM Tris-HCl (pH 8.0)

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  1. doi:10.1101/pdb.rec10432 Cold Spring Harb Protoc 2006: pdb.rec10432-
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