Recipe

Stacking gel buffer (4X)

Tris base

HCl

Dissolve 60.5 g of Tris base in 850 mL of H2O. Adjust the pH to 6.8 with 6 M HCl. Readjust the pH to 6.8 at room temperature. Add H2O to 1000 mL. Store the buffer at 4°C.

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  1. doi:10.1101/pdb.rec10473 Cold Spring Harb Protoc 2006: pdb.rec10473-
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