Recipe

10X Running buffer

Dissolve 30.0 g of Tris base, 144.0 g of glycine, and 10.0 g of SDS in 1000 ml of H2O. The pH of the buffer should be 8.3 and no pH adjustment is required. Store the running buffer at room temperature and dilute to 1X before use.

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  1. doi:10.1101/pdb.rec10475 Cold Spring Harb Protoc 2006: pdb.rec10475-
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