Recipe

Lysis buffer for Drosophila embryo extract preparation

 
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5 mM dithiothreitol

30 mM HEPES-KOH (pH 7.4)

2 mM magnesium acetate

1 mg/ml Pefabloc SC (Boehringer Mannheim)

100 mM potassium acetate

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  1. doi:10.1101/pdb.rec10487 Cold Spring Harb Protoc 2006: pdb.rec10487-
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