NGM worm culturing media

NaCl, 3 g

agar, 17 g

peptone, 2.5 g

5 mg/ml cholesterol, 1 ml

1 M CaCl₂, 1 ml

1 M MgSO₄, 1 ml

1 M K₂HPO₄/KH₂PO₄ (pH 6), 25 ml

Dissolve 3 g of NaCl, 17 g of agar, 2.5 g of peptone, and 1 ml of 5 mg/ml cholesterol in 1 liter of H₂O. After autoclaving, add 1 ml of 1 M CaCl₂, 1 ml of 1 M MgSO₄, and 25 ml of potassium phosphate buffer (pH 6). Prepare plates and seed E. coli as a food source for the nematodes.