Recipe

10x M9 buffer

 
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KH2PO4, 30 g

K2HPO4, 60 g

NaCl, 50 g

1 M MgSO4, 10 ml

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Dissolve the KH2PO4, K2HPO4, and NaCl in 1 liter of H2O and autoclave. After autoclaving, add 10 ml of 1 M MgSO4.

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  1. doi:10.1101/pdb.rec10496 Cold Spring Harb Protoc 2006: pdb.rec10496-
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