IPG Equilibration buffer

6 M urea

30% (w/v) glycerol

2% (w/v) SDS in 0.05 M IPG Tris-HCl buffer (pH 8.8)

To make 500 ml, combine 180 g of urea, 150 g of glycerol, 10 g of SDS, and 16.7 ml of IPG Tris-HCl buffer. Dissolve in deionized H₂O and adjust the volume to 500 ml. This buffer can be stored at room temperature for up to 2 weeks.