2D PAGE agarose solution

Agarose

Electrode buffer

Bromophenol blue

Suspend 1.0 g of agarose in 90 ml of electrode buffer and 100 ml of deionized H₂O and melt it in a boiling water bath or in a microwave oven. Then add 10 ml of bromophenol blue solution (50 mg of bromophenol blue dissolved in 10 ml of electrode buffer).