MES/Bis-Tris/Tris acetate buffer (50 mM)

39 mM MES

9 mM Bis-Tris

2 mM Tris acetate (pH 5.6)

Dissolve 15.23 g of MES, 3.77 g of Bis-Tris, and 0.725 g of Tris acetate in 2 liters of H₂O. Buffer sachets of this formulation are available as Gradiflow Buffer, MES/Bis-Tris/Tris Acetate, 50 mM (Gradipore). Use of these sachets is strongly recommended to provide reliable separation performance.