Recipe

Trichloroacetic acid (TCA) in acetone

Trichloroacetic acid (TCA), 100%

Acetone

Prepare by adding 10 ml of 100% (w/v) TCA to 90 ml acetone and mixing. Because TCA is extremely hygroscopic, and thus difficult to weigh accurately, prepare a 100% (w/v) TCA stock solution by adding 227 ml of H2O to a 500-g bottle of TCA (this can then easily be adjusted to any desired TCA concentration by dilution with acetone).

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  1. doi:10.1101/pdb.rec10513 Cold Spring Harb Protoc 2006: pdb.rec10513-

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