Recipe

Tris-Sucrose

 
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10% (w/v) sucrose

50 mM Tris-Cl (pH 8.0)

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Sterilize the solution by passing it through a 0.22-μm filter, and store it at room temperature. Solutions containing sucrose should not be autoclaved since the sugar tends to carbonize at high temperatures.

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  1. doi:10.1101/pdb.rec418 Cold Spring Harb Protoc 2006: pdb.rec418-
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