Recipe

Deionized formamide

Formamide

8% gel resin (equivalent of Dowex XG8 mixed bed resin)

Deionize the formamide by stirring with an 8% gel resin in a glass beaker for 1 h on a magnetic stirrer. Remove the resin by filtering twice through Whatman No. 1 paper. Store deionized formamide in small aliquots under nitrogen (to prevent oxidation) at −70°C.

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  1. doi:10.1101/pdb.rec436 Cold Spring Harb Protoc 2006: pdb.rec436-
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