Recipe

Trace metals stock solution

4.84 g Na2EDTA•2H2O

2.30 g ZnSO4•7H2O

1.11 g H3BO3

0.51 g MnCl2•4H2O

0.17 g CoCl2•6H2O

0.15 g CuSO4•5H2O

0.10 g (NH4)6Mo7O24•4H2O

To prepare a 10,000X stock solution of trace metals, add the above ingredients to 75 mL of H2O; adjust pH to 6.5 and volume to 100 mL. Freeze aliquots at −20°C.

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  1. doi:10.1101/pdb.rec683 Cold Spring Harb Protoc 2006: pdb.rec683-

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