Recipe

STET

 
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0.1 M NaCl

5% (v/v) Triton X-100

10 mM Tris-Cl (pH 8.0)

1 mM EDTA (pH 8.0)

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Make sure that the pH of STET is 8.0 after all ingredients are added. There is no need to sterilize STET before use.

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  1. doi:10.1101/pdb.rec8027 Cold Spring Harb Protoc 2006: pdb.rec8027-
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