EDTA

EDTA (ethylenediamenetetraacetic acid)

NaOH

To prepare EDTA at 0.5 M (pH 8.0): Add 186.1 g of disodium EDTA•2H₂O to 800 mL of H₂O. Stir vigorously on a magnetic stirrer. Adjust the pH to 8.0 with NaOH (~20 g of NaOH pellets). Dispense into aliquots and sterilize by autoclaving. The disodium salt of EDTA will not go into solution until the pH of the solution is adjusted to ~8.0 by the addition of NaOH.