Recipe

SDS

Also called sodium dodecyl sulfate or sodium lauryl sulfate. To prepare a 20% (w/v) solution, dissolve 200 g of electrophoresis-grade SDS in 900 mL of H2O. Heat to 68°C and stir with a magnetic stirrer to assist dissolution. If necessary, adjust the pH to 7.2 by adding a few drops of concentrated HCl. Adjust the volume to 1 L with H2O. Store at room temperature. Sterilization is not necessary. Do not autoclave.

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  1. doi:10.1101/pdb.rec8039 Cold Spring Harb Protoc 2006: pdb.rec8039-
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