Recipe

Ammonium acetate

To prepare a 10 M solution in 1 L, dissolve 770 g of ammonium acetate in 800 mL of H2O. Adjust volume to 1 L with H2O. Sterilize by filtration. Alternatively, to prepare a 100-mL solution, dissolve 77 g of ammonium acetate in 70 mL of H2O at room temperature. Adjust the volume to 100 mL with H2O. Sterilize the solution by passing it through a 0.22-μm filter. Store the solution in tightly sealed bottles at 4°C or at room temperature. Ammonium acetate decomposes in hot H2O and solutions containing it should not be autoclaved.

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  1. doi:10.1101/pdb.rec8067 Cold Spring Harb Protoc 2006: pdb.rec8067-

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