TM

MgSO₄•7H₂O, 2 g

1 M Tris-Cl (pH 7.5), 50 ml

H₂O, to 1 liter

Sterilize the buffer by autoclaving for 20 minutes at 15 psi (1.05 kg/cm²) on liquid cycle. After the solution has cooled, dispense 50-ml aliquots into sterile containers. TM may be stored indefinitely at room temperature.