Pancreatic DNase I

1 mg/ml Pancreatic DNase I

50 mM NaCl

10 mM Tris-Cl (pH 7.5)

1 mM MgCl₂

Dissolve 2 mg of crude pancreatic DNase I (Sigma or equivalent) in 1 ml of 50 mM NaCl, Tris-Cl (pH 7.5), 1 mM MgCl₂.

When the DNase I is dissolved, add 1 ml of glycerol to the solution and mix by gently inverting the closed tube several times. Take care to avoid creating bubbles and foam. Store the solution in aliquots of -20°°C.