TB

yeast extract, 24 g

For solid medium, please see Media Containing Agar or Agarose.

deionized H₂O, to 900 ml

glycerol, 4 ml

tryptone, 12 g

Shake until the solutes have dissolved and then sterilize by autoclaving for 20 minutes at 15 psi (1.05 kg/cm ²) on liquid cycle. Allow the solution to cool to 60°C or less, and then add 100 ml of a sterile solution of 0.17 M KH₂PO₄, 0.72 M K₂HPO₄. (This solution is made by dissolving 2.31 g of KH₂PO₄ and 12.54 g of K₂HPO₄ in 90 ml of deionized H₂O. After the salts have dissolved, adjust the volume of the solution to 100 ml with deionized H₂O and sterilize by autoclaving for 20 minutes at 15 psi [1.05 kg/cm ²] on liquid cycle.)