Recipe

Acrylamide:bisacrylamide

Stock solutions other than 29:1 (% w/v) acrylamide:bisacrylamide can be used to cast polyacrylamide gels. However, it is then necessary to recalculate the appropriate amount of stock solution to use. Gels can be cast with acrylamide solutions containing different acrylamide:bisacrylamide (cross-link) ratios, such as 19:1 and 37.5:1, in place of the 29:1 ratio recommended here. The mobility of DNA and dyes in such gels will be different from those given in this protocol. Warning: Wear gloves while working with acrylamide.

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  1. doi:10.1101/pdb.rec8236 Cold Spring Harb Protoc 2006: pdb.rec8236-
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