Microtiter cell lysis buffer

0.5% (w/v) Sarkosyl

10 mM EDTA (pH 8.0)

10 mM NaCl

10 mM Tris-Cl (pH 7.5)

Add proteinase K to the lysis buffer to a final concentration of 1 mg/ml just before use.

Sarkosyl, an anionic detergent, is usually supplied by the manufacturer as a 30% solution in H₂O. It is less prone than SDS to precipitate from solutions of high ionic strength. However, it is also a less effective detergent than SDS.