Recipe

SNET

 
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400 mM NaCl

1% (w/v) SDS

20 mM Tris-Cl (pH 8.0)

5 mM EDTA (pH 8.0)

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Sterilize the solution by filtration through a 0.45-μm nitrocellulose filter. Store the sterile solution in 50-ml aliquots at room temperature.

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  1. doi:10.1101/pdb.rec8283 Cold Spring Harb Protoc 2006: pdb.rec8283-
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