Recipe

SSC

For a 20X solution: Dissolve 175.3 g of NaCl and 88.2 g of sodium citrate in 800 mL of H2O. Adjust the pH to 7.0 with a few drops of a 14 N solution of HCl. Adjust the volume to 1 L with H2O. Dispense into aliquots. Sterilize by autoclaving. The final concentrations of the ingredients are 3.0 M NaCl and 0.3 M sodium citrate.

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  1. doi:10.1101/pdb.rec8297 Cold Spring Harb Protoc 2006: pdb.rec8297-
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