MOPS electrophoresis buffer

0.2 M MOPS (pH 7.0)

20 mM sodium acetate

10 mM EDTA (pH 8.0)

For 10x solution: Dissolve 41.8 g of MOPS in 700 ml of sterile Diethyl pyrocarbonate (DEPC)-treated H₂O. Adjust the pH to 7.0 with 2 N NaOH. Add 20 ml of Diethyl pyrocarbonate (DEPC)-treated 1 M sodium acetate and 20 ml of Diethyl pyrocarbonate (DEPC)-treated 0.5 M EDTA (pH 8.0). Adjust the volume of the solution to 1 liter with Diethyl pyrocarbonate (DEPC)-treated H₂O. Sterilize the solution by passing it through a 0.45-μm Millipore filter, and store it at room temperature protected from light. The buffer yellows with age if it is exposed to light or is autoclaved. Straw-colored buffer works well, but darker buffer does not.