Prehybridization Solution for Northern Hybridization

0.5 M sodium phosphate (pH 7.2)*

1 mM EDTA (pH 7.0)

*0.5 M phosphate buffer is 134 g of Na₂HPO₄⋅7H₂O, 4 ml of 85% H₃PO₄ (concentrated phosphoric acid), H₂O to 1 liter.

7% (w/v) SDS

For dot, slot, and northern hybridization.

*0.5 M phosphate buffer is 134 g of Na₂HPO₄•7H₂O, 4 ml of 85% H₃PO₄ (concentrated phosphoric acid), H₂O to 1 liter.