Recipe

Formamide loading buffer

80% (w/v) deionized formamide

1 mg/mL xylene cyanol (xylene cyanol FF)

1 mg/mL bromophenol blue

10 mM EDTA (pH 8.0)

Purchase a distilled deionized preparation of formamide and store in small aliquots under nitrogen at −20°C.

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  1. doi:10.1101/pdb.rec8371 Cold Spring Harb Protoc 2006: pdb.rec8371-
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