Recipe

DD-PCR reverse transcriptase buffer

 
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250 mM Tris-Cl (pH 8.3)

375 mM KCl

15 mM MgCl2

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Autoclave the 5x buffer for 10 minutes at 15 psi (1.05 kg/cm2) on liquid cycle. Divide the sterile buffer into aliquots. Store the aliquots at -20°C.

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  1. doi:10.1101/pdb.rec8407 Cold Spring Harb Protoc 2006: pdb.rec8407-
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