Recipe

Random primer buffer

 
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250 mM Tris (pH 8.0)

25 mM MgCl2

100 mM NaCl

10 mM dithiothreitol

1 M HEPES (adjusted to pH 6.6 with 4 M NaOH)

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To prepare 5x buffer, use a fresh dilution in H2O of 1 M dithiothreitol stock, stored at -20°C. Discard the diluted dithiothreitol after use.

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  1. doi:10.1101/pdb.rec8431 Cold Spring Harb Protoc 2006: pdb.rec8431-
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