Recipe

Imidazole buffer

 
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500 mM imidazole•Cl (pH 6.4)

180 mM MgCl2

50 mM dithiothreitol

1 mM spermidine HCl

1 mM EDTA (pH 8.0)

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For a 10x buffer.

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  1. doi:10.1101/pdb.rec8451 Cold Spring Harb Protoc 2006: pdb.rec8451-
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