Recipe

Oligonucleotide elution buffer

0.5 M ammonium acetate

10 mM magnesium acetate

Some investigators include 0.1% (w/v) SDS in the oligonucleotide elution buffer. This is not advisable if the eluted oligonucleotide is to be purified by Sep-Pak C18 chromatography.

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  1. doi:10.1101/pdb.rec8486 Cold Spring Harb Protoc 2006: pdb.rec8486-

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