EDTA-Tris
To prepare EDTA-Tris, at 0.5 M, pH 6.0, add 0.1 mole of EDTA to 60 ml of H2O. While stirring the solution, slowly add Tris base (powder) until the pH of the solution reaches 6.0. By this stage, the concentration of Tris will be approx. 1.2 M. Adjust the volume of the solution to 200 ml with H2O.
