Recipe

Oligonucleotide hybridization solution

 
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6x SSC (or 6x SSPE)

0.05 M sodium phosphate (pH 6.8)

1 mM EDTA (pH 8.0)

5x Denhardt's solution

100 μg/ml denatured, fragmented salmon sperm DNA

100 mg/ml dextran sulfate

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  1. doi:10.1101/pdb.rec8501 Cold Spring Harb Protoc 2006: pdb.rec8501-
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