Recipe

Dithiothreitol (DTT) (1 M)

DTT (DL-dithiothreitol, anhydrous m.w. = 154.25)

Dissolve 1.5 g of DTT in 8 mL of H2O. Adjust the total volume to 10 mL, dispense into 1-mL aliquots, and store them in the dark (wrapped in aluminum foil) at -20°C (indefinitely). Do not autoclave DTT or solutions containing it.

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  1. doi:10.1101/pdb.rec8519 Cold Spring Harb Protoc 2006: pdb.rec8519-
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