Denhardt’s reagent (50X)
| Reagent | Amount to add | Final concentration (50X) |
|---|---|---|
| Ficoll 400 | 1 g | 1% (w/v) |
| Polyvinylpyrrolidone (PVP) | 1 g | 1% (w/v) |
| Bovine serum albumin (Fraction V) | 1 g | 1% (w/v) |
| H2O | to 100 mL (final volume) | -- |
| Dissolve the components in the H2O. Filter to sterilize and remove particulate matter. Divide into aliquots and store at -20°C. | ||
| Usually, the 50X stock solution is diluted 10-fold into prehybridization buffer (e.g., 6X SSC or 6X SSPE containing 1.0% SDS and 100 μg/mL denatured salmon sperm DNA). | ||
