Recipe

Alkaline Gel-loading Buffer

300 mM NaOH

6 mM EDTA

18% (w/v) Ficoll (Type 400, Pharmacia)

0.15% (w/v) bromocresol green

0.25% (w/v) xylene cyanol

For a 6x buffer.

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  1. doi:10.1101/pdb.rec8570 Cold Spring Harb Protoc 2006: pdb.rec8570-

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