Recipe

TPE

108 g Tris base

15.5 ml of 85% (1.679 g/ml) phosphoric acid

40 ml of 0.5 M EDTA (pH 8.0)

The 1x working solution is 90 mM Tris-phosphate/2 mM EDTA.

Prepare a 10x stock solution in 1 liter of H2O

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  1. doi:10.1101/pdb.rec8645 Cold Spring Harb Protoc 2006: pdb.rec8645-

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